http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113174338-A

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filingDate 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_daa877e7b784178ee97eafff8ed23730
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publicationDate 2021-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113174338-A
titleOfInvention A kind of black fungus tissue separation medium and application thereof
abstract The invention relates to the technical field of microbial culture, in particular to a black fungus tissue separation medium and application thereof. In the present invention, the PDA culture medium is improved by adding camphor wood to the raw materials, and boiling and extracting to obtain camphor wood PDA culture. Based on further research on the medium, it was found that the medium has obvious effects of promoting the growth of black fungus mycelium and inhibiting mucor fungi, and for this reason, it can be used for the tissue separation of black fungus. When using the medium for the separation of Auricularia auriculae tissue, no disinfection step is required, only the ear pieces are cleaned with water, and then the ear pieces are inserted into the medium for culture, and the tissue separation of the black fungus can be completed. The method is particularly suitable for separating Auricularia auricula strains in field tissues with poor experimental conditions, and is a simple, efficient and fast method for separating Auricularia auricula strains and tissues, which can effectively reduce the difficulty of separating Auricularia auricula in the field and improve the separation of strains. efficiency.
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