| abstract |
The invention relates to a chemiluminescence immunoassay kit for detecting CD47, and a preparation method and application thereof. The chemiluminescence immunoassay kit comprises: the kit comprises a microporous plate, a CD47 standard substance gradient solution, a sample diluent, a horseradish peroxidase-labeled CD47 monoclonal antibody 2, an enzyme-labeled diluent, a 20X concentrated washing solution, a luminescent solution A and a luminescent solution B; according to the chemiluminescence immunoassay kit for detecting CD47, the intensifier HIOP is added into the luminescent liquid B, and is a phenolic compound 4-hydroxy-4' -iodobiphenyl with an active substituent at the para-position, compared with the traditional intensifier, the intensifying effect is better, the detection limit is reduced, the linear range is widened, and the sensitivity, the precision and the detection speed of the chemiluminescence immunoassay kit for detecting CD47 are obviously improved. |