| abstract |
The invention belongs to the technical field of biological detection, and provides a fluorescence composite amplification system of 55 autosomal loci, a kit and application thereof, wherein the kit can simultaneously amplify and analyze 30 SNP loci and 25 autosomal Indel loci 3. The invention adopts five-color fluorescence labeling, the size of the amplified product is between 80 and 400bp, the operation is simple, higher accumulated individual identification power and accumulated non-father exclusion rate are provided, and the invention has the laboratory-level sample pollution alarm capability. |