http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113151416-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18086029ee284d70cc9ce46d117d4ff3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2021-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ab8815e4e91f3fb698c75b7dd0a4cc2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_413a3c7e3e7226a0eb79cfebfd6e8fc1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_277b434030e3fc3f3f3a19080b2a2eb9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc834269763c3493e1ad78141532cacb |
| publicationDate | 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113151416-A |
| titleOfInvention | A droplet digital detection method for detecting mutant nucleic acid |
| abstract | The present invention provides a droplet digital detection method for detecting mutant nucleic acid, the method comprising the following steps: (1) preparing a mixture required for a loop-mediated isothermal amplification reaction according to a target mutant nucleic acid; (2) combining the steps (1) The obtained reaction mixture is used as the dispersed phase, and the oil phase is used as the continuous phase, which are respectively injected into the microfluidic chip to generate monodisperse droplets; (3) The microfluidic chip described in step (2) is placed at a constant temperature (4) Detecting and analyzing the fluorescent signal of the droplets in the microfluidic chip after the reaction in step (3). The method of the invention can be completed without complex chips, controllable temperature control equipment and other high-cost equipment, and provides a simple process, stable signal, high sensitivity and accuracy for gene diagnosis and gene therapy. means of digital analysis of mutant nucleic acids. |
| priorityDate | 2021-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 152.