http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113151233-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01084 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113151233-B |
| titleOfInvention | Nitrile hydratase lysine mutant HBA-K2H2, encoding gene and application |
| abstract | The present invention discloses a nitrile hydratase mutant, in particular to a nitrile hydratase lysine mutant HBA-K2H2 and its encoding gene, a plasmid and recombinant bacteria containing the mutant encoding gene, and Application of the nitrile hydratase lysine mutant HBA-K2H2 in catalyzing organic nitrile compounds to prepare amide compounds. The amino acid sequence of the mutant HBA-K2H2 is shown in SEQ ID NO. 1, its optimum pH is 9.0, and its optimum temperature is 35°C. Compared with the wild enzyme HBA, the alkaline activity of the mutant nitrile hydratase HBA-K2H2 has been improved, and it can be used in the biocatalytic production of acrylamide, nicotinamide and other high value-added amide compounds, chemical fiber surface modification and alkali Biotechnology fields such as sewage treatment. |
| priorityDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.