http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113151211-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-18 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2021-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113151211-B |
| titleOfInvention | Alpha-1, 3-fucosyltransferase mutant and application thereof in preparing 3-fucosyllactose |
| abstract | The invention relates to the technical field of biology, and particularly discloses an alpha-1, 3-fucosyltransferase mutant and application of the mutant in preparation of 3-fucosyllactose. The alpha-1, 3-fucosyltransferase mutant has at least 90 percent of nucleotide sequence identity with SEQ ID NO. 1, and comprises mutation at one or more positions, wherein the mutation site comprises at least one of the 46 th, 128 th, 129 th or 132 th positions of the SEQ ID NO. 1 or corresponding positions, and the mutation is to replace, insert or delete one or more amino acid residues at the mutation site. The alpha-1, 3-fucosyltransferase mutant prepared by the invention can synthesize 3-fucosyllactose in genetically engineered cells with the yield of 10.96g/L, which is 64.5 times higher than that of a wild strain FutA, and the effect of improving the yield of 3-fucosyllactose is obvious. |
| priorityDate | 2021-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.