http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113151176-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 |
| filingDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113151176-B |
| titleOfInvention | A method for enrichment of circulating tumor cells based on diethylaminoethyl agarose microspheres and spheroids |
| abstract | The invention discloses a circulating tumor cell enrichment method based on diethylaminoethyl agarose microspheres and spherical red blood cells, and belongs to the technical field of biomedicine. The method of the present invention includes the following steps: adding spherical red blood cells with surface-modified FA, RGD or tumor cell targeting antibodies to the sample to be analyzed containing CTCs and leukocytes, incubating the red blood cells to wrap the tumor cells, then adding DEAE agarose microspheres, and incubating The leukocytes were adsorbed on DEAE agarose microspheres, and the DEAE agarose microspheres adsorbed with leukocytes were precipitated by natural sedimentation or centrifugation, and the supernatant was collected to obtain purified circulating tumor cells after the leukocytes were removed. The invention provides a simple and feasible technical solution for enriching circulating tumor cells based on different densities. The involved material preparation method is very simple, the steps of the enrichment process are simple, and the enrichment effect is very ideal, which can be designed based on the invention. Automated equipment is used in clinical testing. |
| priorityDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.