| abstract |
The present invention relates to a dividing solution for bovine embryo dividing and a bovine embryo dividing method. The dividing solution comprises: 9.39 g of dipotassium hydrogen phosphate, 3.5 g of potassium dihydrogen phosphate, 68 g of sucrose, 12 g of povidone K30, and water for injection to 1000 ml. The bovine embryo segmentation method includes the following steps: collecting oocytes and making them mature in vitro; incubating the sperm suspension with the mature oocytes to complete in vitro fertilization; culturing the fertilized embryos to morula, and selecting the morula; The morula is transferred into the dividing droplet and placed under an inverted microscope to divide the embryo into two parts; the divided half embryo is transferred into the embryo culture medium and cultured until the morula develops into an expanded blastocyst that can be used for embryo transfer. Using the embryo dividing solution of the present invention can effectively improve the effect of embryo dividing. |