http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113145079-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1540f8e9b97a0cbe9fd95c84a9dbbfb2 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-28016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-28009 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-103 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-22 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-30 |
filingDate | 2021-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eef358722694f3092c756d5d0b2619a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_355e40ddb107ae6d71f694ca4e01ade7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c64c0d5f927407d669c23b771c3bbee |
publicationDate | 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113145079-A |
titleOfInvention | Novel coronavirus nucleic acid extraction kit and nucleic acid extraction method |
abstract | The invention discloses a novel coronavirus nucleic acid extraction kit and a nucleic acid extraction method, belonging to the technical field of nucleic acid extraction, wherein the kit comprises a suspension prepared from novel biological nano magnetic beads, and the preparation method of the novel biological nano magnetic beads comprises the following steps: mixing Fe 3 O 4 @SiO 2 The nano magnetic beads are reacted with a modified silane coupling agent obtained by modifying chloromethyl trimethoxy silane with urea. The method for extracting the novel coronavirus nucleic acid by using the kit comprises the following steps: adding lysis solution into the sample, adding washing solution after the sample is lysed at room temperature, oscillating and centrifuging, taking supernate, adding novel biological nano magnetic bead suspension into the supernate, and adsorbing at room temperature; removing supernatant after adsorptionAdding a washing solution and mixing uniformly; standing, removing supernatant and air drying; after air drying, adding an eluent to elute at room temperature to obtain an RNA solution. The kit can extract high-purity and high-concentration nucleic acid, and the extracted nucleic acid is not easy to degrade. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114438074-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115532221-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114934041-A |
priorityDate | 2021-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.