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filingDate 2021-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113136402-B
titleOfInvention A method for overexpressing PCNP gene and its application in the treatment of human liver cancer
abstract The invention discloses a method for overexpressing PCNP gene and its application in treating human liver cancer. The method for overexpressing PCNP gene specifically includes: amplifying human PCNP gene, linearizing GV230 vector, constructing GV230-PCNP vector, PCR and Sequencing identification, transient transfection, stable transfection and other steps. After this method overexpresses the PCNP gene in human liver cancer, the growth, migration and invasion of human liver cancer can be significantly inhibited. The invention can specifically increase the expression level of PCNP gene in human liver cancer; in vitro experiments show that overexpression of PCNP gene in human liver cancer can significantly inhibit the growth, migration and invasion of human liver cancer, and promote the apoptosis of human liver cancer.
priorityDate 2021-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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