http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113136378-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-0104 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
| filingDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113136378-B |
| titleOfInvention | Rhamnosidase TpeRhha mutant and preparation method and application thereof |
| abstract | The invention discloses a rhamnosidase TpeRha mutant and a preparation method and application thereof, relates to the technical field of biological medicines, and relates to any one of a mutant TpeRha-R369A, a mutant TpeRha-W512A and a mutant TpeRha-K579A; the mutant TpeRhha-R369A has an amino acid sequence shown as SEQ ID NO: 1, arginine at position 369 of the tpeRha enzyme is mutated into alanine; the mutant TpeRhha-W512A is obtained by mutating tryptophan with the amino acid sequence of the 512 th site of the TpeRhha enzyme into alanine. The invention constructs the combined mutant of alpha-L-rhamnosidase TpeRhha by site-directed mutagenesis technology, and prepares the icariside I composition by catalyzing and converting multi-component flavonoid glycoside in epimedium total flavone, and the enzymolysis time is short and the conversion rate is high. |
| priorityDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 510.