http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113136359-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 |
| filingDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113136359-B |
| titleOfInvention | A method for observing subcellular localization and protein interaction using creeping bentgrass protoplasts |
| abstract | The present invention relates to a method for observing subcellular localization and protein interaction by using creeping bentgrass protoplasts. The method provided by the invention includes: cutting the stem and leaf sheath tissue of the creeping bentgrass into pieces, transferring it to a culture dish containing an enzyme solution to avoid light for enzymatic hydrolysis; filtering and centrifuging to obtain the protoplast of the creeping bentgrass stem. Among the protoplasts of the creeping bentgrass glume stems prepared by the invention, 97% of the protoplasts have intact characters and no damage; the transformation rate of the protoplasts transformed by the target gene can reach 65%. When using the protoplast of the creeping bentgrass stem provided by the present invention for subcellular localization, it is accurately determined that the NYC1 gene is located in the chloroplast of the creeping bentgrass, and the NAC3 gene is located in the nucleus of the creeping bentgrass cell. The creeping bentgrass stem protoplast provided by the invention can also be used for protein interaction analysis, and provides technical support for the subsequent gene function research of creeping bentgrass. |
| priorityDate | 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.