http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113134079-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_16976617eacab4c618dd0d1586074d78 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-55511 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-04 |
| filingDate | 2020-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_463b3872f70d56cb412e37389bcc7bc7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5bb24e2d83b32f59962eeed6982749dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6017f3e0ef6a74e6b6d4986b4740c9fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd3428903fae48186d3dc616d94ea585 |
| publicationDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113134079-A |
| titleOfInvention | An immunoadjuvant for neutralizing antibodies |
| abstract | The invention discloses an immune adjuvant for neutralizing antibodies and a preparation method thereof, comprising the following steps: dissolving 20 g of Mega-10 in 100 ml of double distilled water to prepare a 20% solution; weighing 1 g of phosphatidylcholine and 1g cholesterol was dissolved in the above solution to make the final concentration reach 10mg/ml respectively; the saponin was dissolved in phosphate buffered saline PBS (pH6.2) to make the effective final concentration reach 100mg/ml; 176ml PBS without saponin , 16ml Mega-10, a mixture of phosphatidylcholine and cholesterol, 8ml saponin solution, mix well, 200W ice bath ultrasound for 10S, interval 10S, repeated 10 times; 25 ℃ gentle mixing 2Hr; put the above solution into 3kDa dialysis In the bag, the PBS solution was dialyzed, and the solution was repeatedly exchanged for more than 10 times to obtain the prepared nano-adjuvant. In the present invention, a unique preparation method is used to generate neutralizing antibodies with high specificity and high titer, which improves the success rate of preparation, effectively reduces the workload, and reduces the difficulty of screening. |
| priorityDate | 2020-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.