http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125728-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46b74dc0a190e06eb47a119bac855d1b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e58ac1319970945335aa848d96652464 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdd04953de6f6338d81c3841c3241ee0 |
| publicationDate | 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113125728-A |
| titleOfInvention | Preparation of enzyme linked immunosorbent assay kit for detecting variegated aflatoxin |
| abstract | The invention relates to an enzyme linked immunosorbent assay kit for detecting the aflatoxin and a detection method thereof, which have the advantages of sensitive, accurate and rapid detection, simple and convenient operation and strong specificity and are suitable for detecting a large number of samples. The kit comprises: the kit comprises an ELISA plate for coating a variegated aflatoxin antigen, a variegated aflatoxin standard substance, a variegated aflatoxin antibody working solution, a variegated aflatoxin enzyme-labeled secondary antibody working solution, a substrate solution A, a substrate solution B, a stop solution, a concentrated diluent and a concentrated washing solution. The principle of the enzyme linked immunosorbent assay kit for detecting the variegated aflatoxin is that a solid phase indirect competitive enzyme linked immunoreaction is carried out, an extracted sample, enzyme-labeled secondary antibody working solution and antibody working solution are added into corresponding micropores of an enzyme label plate, after incubation for a period of time, the substrate solution A and the substrate solution B are added into the plate, a color developing agent presents blue under the action of enzyme, and a stop solution is added, so that the color is changed from blue to yellow. The color depth of the color development is inversely proportional to the content of the aflatoxin in the standard or the sample. The method can be directly used for detecting the residual quantity of the aflatoxin in the dairy product. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114806888-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114806888-B |
| priorityDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.