http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125717-A

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filingDate 2021-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cea274d9817412eed163746fc513a2e
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publicationDate 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113125717-A
titleOfInvention Method and device for detecting concentration of salmonella in food based on micro-fluidic chip
abstract The invention discloses a method and a device for detecting the concentration of salmonella in food based on a microfluidic chip, wherein the device comprises a microfluidic pipeline area, a screen printing electrode layer, an electronic refrigerating sheet layer, a bimorph layer and a PCB circuit board layer from top to bottom, a glass substrate at the bottom of the microfluidic pipeline area is respectively provided with a sample inlet, a carbonic acid liquid storage chamber, an ethanol liquid storage chamber, a nano gold particle solution liquid storage chamber, a lipopolysaccharide antibody solution chamber, a BSA solution liquid storage chamber and a PBS buffer solution liquid storage chamber, and the sample inlet is sequentially connected in series with a first micro-mixing cavity, a second micro-mixing cavity and a first through hole through a pipeline; firstly, modifying a screen printing electrode, measuring a first impedance value of the surface of a working electrode, then dripping salmonella sample liquid into a sample inlet, dripping liquid into the surface of a working area, measuring a second impedance value of the surface of the working electrode, finally comparing the two impedance values with a relational graph, simulating to obtain a concentration value of salmonella, and adopting a multi-pipeline microfluidic chip to quickly, simply and conveniently detect the salmonella with high sensitivity.
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