http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113122515-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6967bba8150e0b546153ebb0fbc0eec9 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y107-03003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0048 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
filingDate | 2020-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bd671e6b479807f438d3ab470ab9912 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_585205b5fd61e6bd9b02ad671c534a2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_622260a7cd062eb4188baf90f2141505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_20835d1d28b0dbd944f819a79462fe86 |
publicationDate | 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113122515-A |
titleOfInvention | A kind of fermentation method of genetically engineered bacteria recombinant uricase |
abstract | The invention belongs to the field of recombinant DNA technology for producing genetically engineered drugs, and specifically discloses a fermentation medium and a fermentation method for recombinant uricase of genetically engineered bacteria. The improvement of the method makes the fermentation density OD 600nm of recombinant uricase produced by Escherichia coli fermentation reach 110-130. Compared with low-density fermentation, the bacterial density is increased by more than 4 times; the yield of recombinant uricase is as high as 5.5g/L, and the recombinant uricase protein The expression amount accounted for 57% of the total bacterial protein, and the plasmid loss rate was low. The high-density fermentation of the recombinant uricase of the invention improves the production efficiency and has better application prospects. |
priorityDate | 2020-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 91.