http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113122499-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_65682d3e437c225d79b93953b4b26392 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0622 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
| filingDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5c223c1e444a6560b517c869e8fc2eb |
| publicationDate | 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113122499-A |
| titleOfInvention | A method for primary culture of microglia that increases yield |
| abstract | The invention relates to a microglia primary culture method capable of improving yield, and belongs to the technical field of primary cell culture. The cell culture method of the invention is as follows: taking animals, sterilizing with alcohol, taking the cerebral cortex in the brain tissue, crushing, placing in trypsin for enzymolysis, and centrifuging to obtain microglia single cells. Resuspend single microglia cells in DF12 microglia medium, incubate the culture, and change the medium; repeat the operation for 7-14 days. The microglia were shaken at 37°C at 200 rpm for 1-3 h, and finally centrifuged to obtain primary microglia. During the culture of primary microglia, 25-100ng/mL M-CSF solution was added, and the cells were incubated for 24h-72h to obtain microglia. The invention can remarkably improve the productivity of microglia cells, retain the functions of microglia phagocytosis and the like, and can be used to study the functions of cell phagocytosis, inflammation and the like. |
| priorityDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.