http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113121672-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-95 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113121672-B |
| titleOfInvention | Soluble prokaryotic expression and purification method and application of feline interferon gamma |
| abstract | The invention provides a soluble prokaryotic expression and purification method and application of feline interferon gamma, belonging to the field of biological genetic engineering. The steps included in the method are: (1) analysis of signal peptide sequence and physicochemical properties of FeIFN-γ protein; (2) construction of prokaryotic expression plasmid pET28a-SUMO-FeIFN-γ; (3) induced expression of BL21 bacteria containing recombinant plasmid and soluble analysis of recombinant protein ; ⑷ Screening for optimal expression conditions of SUMO-FeIFN-γ fusion protein; ⑸ Mass expression and purification of SUMO-FeIFN-γ fusion protein; ⑹ Digestion and purification of SUMO-FeIFN-γ fusion protein. The present invention clones a mature protein gene sequence that does not contain a signal peptide, constructs a prokaryotic expression plasmid, and transforms BL21 competent cells for expression and purification, and uses a pET28a-SUMO expression vector with a SUMO solubilizing tag to realize the target protein The soluble expression of exogenous protein has a high level of soluble expression, has high biological activity, and the purification steps are simple, laying the foundation for the later development of antiviral drugs. |
| priorityDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.