abstract |
The invention relates to an extraction method for simultaneously preparing polysaccharides and saponins from ginseng stems and leaves. The method includes the following steps: 1) taking ginseng stems and leaves, adding water to decoct, filtering the decoction, and combining the filtrates; 2) adding D101 macroporous resin to the filtrate Column, collect sample effluent, concentrate, dry, obtain ginseng stem and leaf polysaccharide, 3) column is further eluted with 10-35% ethanol solution, collect eluate and concentrate and dry, obtain low-content ginseng stem and leaf saponins; 4) The column is further washed with water until colorless, and then eluted with 55-65% ethanol solution. The ethanol eluent is collected from the time when the sugar content of the effluent reaches more than 10%. After the eluent is concentrated, 8-9 times the weight of 90%-95% is added. The ethanol solution was placed, and the precipitate was precipitated. After the precipitate was filtered off, the filtrate was concentrated and dried to obtain the total saponins of ginseng stems and leaves. |