http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113106066-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-405 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 2021-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113106066-B |
| titleOfInvention | A kind of sarcoma cell culture medium and method for producing Matrigel stock solution in vitro by using the culture medium |
| abstract | The invention discloses a sarcoma cell culture medium and a method for producing Matrigel stock solution in vitro by using the culture medium. The sarcoma cell culture medium comprises: DMEM/F12 basal medium, 1.5-2.5v/v% FBS, 0.5-1.5v/v %ITS, sodium pyruvate 0.5-1.5mM, hydrocortisone 20-40nM, 3-6ng/ml FGF2, EGF 40-60ng/ml and Hippo inhibitor. When using this medium to produce Matrigel stock solution in vitro, the mouse sarcoma cells are mixed with 3D bioscaffold in vitro. The pore size of the 3D bioscaffold can make the mouse sarcoma cells maintain their three-dimensional growth state, and then the mouse sarcoma cells are cultured and expanded. Increase, prepare Matrigel stock solution. The preparation process time is short, the amount of mice used is small, and the variability between batches can be greatly reduced. |
| priorityDate | 2021-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.