http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113101992-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0647 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0819 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0487 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-50273 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2021-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113101992-B |
| titleOfInvention | Protein synthesis screening method |
| abstract | The invention discloses a protein synthesis screening method. The invention also discloses a protein screening chip which comprises a liquid flow layer, a pneumatic control layer and an elastic membrane, wherein the elastic membrane is arranged between the liquid flow layer and the pneumatic control layer. The screening method comprises the following steps: enabling the N-terminal sequence fragment to be screened, the target gene fragment and the connecting mixed solution to perform a connecting reaction in a circulating liquid pipeline; inputting the amplification mixed solution into a circulating liquid flow pipeline, and carrying out amplification reaction; and inputting the mixed solution of the cell-free protein expression into a circulating liquid flow pipeline, carrying out cell-free protein expression reaction, and then carrying out enrichment and purification treatment to obtain the target protein. The rapid and convenient protein translation initiation region screening method integrates target gene template optimization, amplification, protein expression, purification and detection, is simple and convenient to operate, and can complete screening of the optimal carrier of the target protein within one day so as to improve the yield of the target protein. |
| priorityDate | 2021-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.