http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113061176-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-523 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65b0ef62608e4f95a6b05fa2b5dca7ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd139eda6a985110f6edbf3da71da307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ac6112fa001dc34fc2106aa5b18d5e9 |
publicationDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113061176-A |
titleOfInvention | A grass carp antibacterial protein LECT2 and its preparation method and application |
abstract | The invention discloses a grass carp antibacterial protein LECT2 and a preparation method and application thereof, belonging to the technical field of genetic engineering, wherein the amino acid sequence of the grass carp antibacterial protein LECT2 is shown in SEQ ID NO: 1. The present invention adopts Escherichia coli BL21 (DE3) Rosetta to express grass carp antibacterial protein LECT2, and obtains LECT2 recombinant protein through Ni 2+ -TED agarose column purification, enterokinase digestion and ion exchange chromatography purification, and finds that LECT2 The protein has significant bactericidal effect on both gram-negative bacteria and gram-positive bacteria, and can be used to prepare broad-spectrum antibacterial agents or antibacterial drugs to protect grass carp from bacterial infection. By injecting it into the infected grass carp, the resistance of the grass carp to bacterial infection is significantly enhanced, the survival rate of the grass carp is improved, and the bacterial load in each tissue is reduced, which has high application value. |
priorityDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 151.