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filingDate 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2db122fcb3c033bb73ee52e27a6746bf
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publicationDate 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113049826-A
titleOfInvention Rapid synchronous multiple detection method and kit for total number of vibrio parahaemolyticus and bacteria
abstract A method and a detection kit for rapidly and synchronously detecting the total number of vibrio parahaemolyticus and bacteria in a sample in a multiplex manner are disclosed, wherein the total number of the bacteria is marked by using a red fluorescent probe capable of marking all the bacteria, so that the bacteria in the sample can be distinguished from other background particles: meanwhile, a green fluorescent probe is used for distinguishing vibrio parahaemolyticus and non-vibrio parahaemolyticus in the sample through a vibrio parahaemolyticus antibody crosslinked by a chemical group; and simultaneously counting red and green fluorescent signals by a flow analyzer to realize synchronous detection of the total number of vibrio parahaemolyticus and bacteria. The method can simultaneously detect the total number of the vibrio parahaemolyticus and the total number of the bacteria, is simple and convenient to operate, consumes short time, and can finish detection in 0.5h for most samples.
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