http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113049697-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1fd75437d55adfbfaecf791a6b6783ff |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-027 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 |
| filingDate | 2021-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_916409d6c1c011d703a71ab29efa10eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5b4bc46a1e459085198e3174765f2ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dff95ece7a50f7991ed21b19e85b77f3 |
| publicationDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113049697-A |
| titleOfInvention | Method and kit for simultaneously detecting activity of aldosterone and renin in blood sample |
| abstract | The invention discloses a method and a kit for simultaneously detecting activity of aldosterone and renin in a blood sample, which comprises a sample pretreatment step and a sample injection detection step, wherein the sample pretreatment step comprises the following steps: adding an incubation buffer solution into a blood sample, incubating for a set time, adding a pH value regulator, purifying to obtain a treatment solution, drying the treatment solution by using nitrogen, and re-dissolving by using a mobile phase to obtain a solution to be detected; the pH value regulator is formic acid, ammonia water or ammonium acetate; and detecting by adopting a liquid chromatography-tandem mass spectrometry method. The optimization direction of the method is that on one hand, aiming at the physical property of angiotensin I, the pretreatment process is optimized, 10-100 mM ammonium acetate is used as a pH value regulator, so that the protonation dissociation of the angiotensin I in a solution to be detected is reduced, the recovery rate of the angiotensin I in SPE treatment is improved, and the detection sensitivity of substances is greatly improved; on the other hand, after SPE extraction, nitrogen blowing operation is added, and sample loading is carried out after redissolution, so that the detection signal is improved. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115453001-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116106466-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116106466-B |
| priorityDate | 2021-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 99.