| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53c911e75ea09dd4c2705db5306dae77 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate |
2019-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40082908623359aa22cfa0045076c6a0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a232ac4a6aac1481ff509362154e363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16438b2ccf0b5bd18ae7b99ac1ec9607
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa8f687f73219288c60b637409799831
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc7398e47e2007081a4367d407a956ef |
| publicationDate |
2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113039285-A |
| titleOfInvention |
Liquid sample workflow for nanopore sequencing |
| abstract |
The present invention relates to methods of characterizing target DNA polynucleotides using Rolling Circle Amplification (RCA) and synthetic single stranded guide rna (sgrna) to identify and cleave the WT version of the target DNA polynucleotide. Also provided are characterization steps based on the use of a transmembrane pore and a DNA translocase that controls the movement of a DNA polynucleotide through the transmembrane pore. Further contemplated is a kit comprising one or more oligonucleotides specific for at least a portion of a target DNA polynucleotide, a sgRNA specific for a WT version of the target DNA polynucleotide, and a sgRNA-guided nucleic acid binding protein. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111154845-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111154845-A |
| priorityDate |
2018-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |