http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113030475-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 |
| filingDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113030475-B |
| titleOfInvention | T cell PD-1 detection method based on cell mitochondrial quality evaluation |
| abstract | The embodiment of the invention provides a T cell PD-1 detection method based on cell mitochondrial quality evaluation, which comprises the following steps: s10: obtaining T cells; s20: adding a fluorescently-labeled monoclonal antibody into the T cells to coil out helper T cells and/or cytotoxic T cells; simultaneously adding a PD-1 antibody which is labeled by fluorescence to obtain a first sample; s30: adding a mitochondrial probe into the first sample, and dividing an MT-group and an MT + group by using the mitochondrial probe as a cell metabolism index to obtain a second sample; s40: detecting the second sample by using a flow cytometer to obtain the expression quantity of PD-1 under different metabolic levels; wherein the MT-group is a low-intensity expression group after the mitochondrial probe is stained, and the MT + group is a high-intensity expression group after the mitochondrial probe is stained. The technical scheme provided by the invention refines PD-1 detection expression through mitochondrial quality evaluation, so that the detection result is more accurate. |
| priorityDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.