http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113025675-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-635 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-605 |
| filingDate | 2021-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113025675-B |
| titleOfInvention | Preparation method of polypeptide |
| abstract | The invention discloses a preparation method of a polypeptide. The preparation method comprises the following steps: constructing an engineering strain with Sumo tag fusion expressing polypeptide gene, and inducing the engineering strain to express the polypeptide soluble; purifying the crude protein containing the polypeptide precursor from the engineering strain; Ulp1 protease is digested to remove the Sumo tag; the cleavage product of Ulp1 protease is purified by the method of acetonitrile combined with heating precipitation or the method of hexafluoroisopropanol precipitation to obtain the polypeptide. The present invention establishes an efficient technology based on soluble recombinant expression of medicinal polypeptide or its precursor, and establishes a simple purification process based on one-step precipitation purification, and further combined with HPLC purification can achieve more than 97% polypeptide purity. |
| priorityDate | 2021-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 136.