abstract |
The invention relates to the field of edible fungi processing, in particular to a low-molecular-weight Bamboo fungus Trophy polysaccharide and a preparation method and application thereof. In the present invention, the dried bamboo fungus tray is degreased, dried and pulverized to obtain dry tray powder; purified water, cellulase, pectinase and papain are added in proportion to adjust pH, and enzymolysis; constant temperature hot water extraction; The extract was filtered, activated carbon decolorized, and deproteinized; centrifuged, concentrated under vacuum and reduced pressure; added ethanol for precipitation, filtration, and centrifugation; Then, it is purified by Sephadex G-200 Sephadex column, eluted, concentrated, and dried to obtain P. chinensis polysaccharide. The invention utilizes the waste suspensory cultivated by bamboo fungus, adopts compound enzyme method to extract low molecular weight polysaccharides, has high extraction rate, small molecular weight of the extracted polysaccharides, high scavenging rate of OH and DPPH, and higher reducing ability to Fe 3+ . Strong, can also inhibit the activity of α-glucosidase and α-amylase. |