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filingDate 2021-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113009152-B
titleOfInvention Glycosylated CD59 enzyme-linked immunoassay kit and preparation method and application thereof
abstract The invention discloses a glycosylated CD59 enzyme-linked immunoassay kit, which consists of an enzyme label plate coated with an anti-gCD 59 antibody, gCD59 standard substance gradient solution, sample diluent, peroxidase-labeled anti-CD 59 monoclonal antibody enzyme conjugate, 20 multiplied concentrated washing solution, a color developing agent, stop solution, a sealing plate film, a sealing bag and a specification, wherein the enzyme label plate, the gCD59 standard substance gradient solution, the sample diluent, the peroxidase-labeled anti-CD 59 monoclonal antibody enzyme conjugate, the 20 multiplied concentrated washing solution, the color developing agent, the stop solution and the sealing plate film are arranged in a box body. The invention also discloses application of the kit in detecting gCD 59-containing biological samples. Experiments prove that the kit disclosed by the invention is high in stability, strong in selectivity, high in detection speed, low in cost, easy to operate, and capable of overcoming the defects of low sensitivity, long operation time and the like in gCD59 detection in the prior art, the operation time is shortened from 400 minutes to 45 minutes, the quantification limit is improved from 18.75pg/mL to 12.5pg/mL, and the kit has a great clinical application prospect.
priorityDate 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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