| abstract |
The invention provides a method for detecting beta-lactam antibiotics in a recombinant hepatitis B vaccine, which adopts a liquid chromatography-mass spectrometer to establish a method for simultaneously detecting 40 beta-lactam antibiotics in the recombinant hepatitis B vaccine for the first time. The method realizes high-flux screening of the residual antibiotics, has the characteristics of high speed, accuracy, high sensitivity and short operation time, and reduces the generation of false positives by adopting an MRM scanning mode. The method has the advantages that the simultaneous qualitative and quantitative analysis of 40 beta-lactam antibiotics in the vaccine provides a new idea for establishing a method for detecting other antibiotic residues in the vaccine, and guarantees are provided for guaranteeing the quality of the vaccine. Meanwhile, the antibiotic residues in different vaccine development stages can be grasped and adjusted in time by detecting the antibiotic residues in different vaccine development stages, so that huge loss caused by unqualified final products is avoided. |