http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113008652-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8537afcedfa27f5a171e292e138abec |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 |
| filingDate | 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7ecac93b22380eefa3f1015bb7d9b33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7884be1d1ddf53dbe099503fda45f271 |
| publicationDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113008652-A |
| titleOfInvention | Method for separating exosome by using TIM-4 functionalized fishbone-shaped microfluidic chip |
| abstract | The invention discloses a method for separating exosomes by using TIM-4 functionalized fishbone-shaped microfluidic chips, which comprises the following steps: collecting the solution to be tested, adding Ca into the solution to be tested 2+ Solution of Ca will be contained 2+ Pumping the liquid to be tested into the TIM-4 functionalized microfluidic chip, capturing the exosomes in the liquid to be tested by the TIM-4 protein in the microfluidic channel, eluting by EDTA eluent, and collecting to obtain the purified exosomes. The capture efficiency of the exosome in the method is about 90%, and the purity is 71.12%, which is superior to the purity of the exosome obtained by ultracentrifugation. In addition, the method has relatively simple steps, the capturing and releasing time is less than 20 minutes, the cost is low, only a small sample amount (20 mu L) is needed, the difference of the PS + exosome content of a tumor patient and that of a healthy person can be detected, and the method can provide a basis for the subsequent detection and diagnosis of the exosome for the tumor. |
| priorityDate | 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.