| abstract |
The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of KDR-CAR-NK cells. The preparation method for constructing KDR-CAR-NK cells provided by the present invention mainly includes the processes of constructing a KDR-CAR sequence, constructing a KDR-CAR sequence vector, constructing a KDR-CAR-NK cell and the like. The KDR-CAR-NK cells constructed by the present invention can express KDR-targeted chimeric antigen receptors, improve the targeting, lethality and vitality of NK cells, so that NK cells can break the immune system of the microenvironment of tumor lesions Suppressing and relieving the immune tolerance of the host has achieved a new breakthrough in adoptive immunotherapy of allogeneic cells. |