http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113005100-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16334 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16222 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16311 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-18122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-18134 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16234 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16134 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16122 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-125 |
| filingDate | 2020-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113005100-B |
| titleOfInvention | Genetic modification method for changing heat stability of enveloped virus, recombinant virus and application thereof |
| abstract | The invention provides a genetic modification method for changing heat stability of a cyst membrane virus, a recombinant virus and application thereof. The method comprises the following steps: comparing the multiple sequences of the enveloped viruses to obtain amino acid mutation which obviously influences the charge value of the adsorption protein of the virus strain to be modified; analyzing the change value of the amino acid mutation on the adsorbed protein charge, and screening the amino acid mutation combination in which the negative charge of the adsorbed protein can be obviously increased or reduced; the genetic operation technology of the virus is applied to carry out mutation transformation on the enveloped virus at the level of transcription plasmid, and the heat-resistant transformed recombinant enveloped virus is rescued and obtained. The invention obviously changes the heat-resistant characteristic of the enveloped virus in an artificial mutation mode for the first time. The invention can be widely applied to heat-resistant modification of enveloped virus vaccine strains, and has wide application prospect in the aspects of researching and developing heat-resistant, safe and efficient enveloped virus vaccines. |
| priorityDate | 2020-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.