| abstract |
The invention belongs to the field of active substance screening and application, and in particular relates to a preparation method and application of a sea cucumber intestinal pro-osteogenic peptide. After the sea cucumber intestine is washed and homogenized, the pH is adjusted to 5-10; after adding protease, it is enzymatically hydrolyzed at 50-60 ℃ for 4-8 hours; after high temperature enzyme inactivation and centrifugation, it is subjected to ultrafiltration through an ultrafiltration membrane with a molecular weight cut-off of 3kDa , and the ultrafiltration liquid is dried to obtain the sea cucumber enterotrophic peptide. The method of the invention has simple process steps, low equipment investment, no environmental pollution, and is suitable for industrial production; the obtained small molecular peptide has strong osteogenic activity, and can be directly eaten as a functional food for promoting bone formation, or as a food. ingredients or health supplements. |