http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112986570-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6818 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2019-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112986570-B |
| titleOfInvention | A method for amino acid sequence determination based on quasi-equivalent double labeling at both ends of the peptide |
| abstract | The present invention relates to a method for de novo sequencing of amino acid sequences of proteins labeled with quasi-isobaric stable isotopes at both ends of a polypeptide. The de novo sequencing method uses trypsin to digest the denatured and reductive alkylated protein to form a peptide segment containing an N-terminal α-amino group and a C-terminal α-carboxyl group. The samples were divided into two parts, one was derivatized with trypsin in 18 O water, and then the N-terminal dimethylation was carried out with formaldehyde; Finally, the two labeled samples were mixed, and the peptides were separated and identified by high performance liquid chromatography-mass spectrometry. The method of the invention not only narrows the window of mass spectrometry, but also can effectively reduce the influence of interference signals, and improves the selection specificity of fragment ions and the accuracy of polypeptide sequencing. At the same time, it is not necessary to distinguish b and y ions, which simplifies the de novo sequencing algorithm of protein amino acid sequences and improves the speed of de novo sequencing. |
| priorityDate | 2019-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.