| abstract |
The invention provides a recombinant escherichia coli for synthesizing an anticancer drug intermediate, and a construction method and application thereof. The recombinant plasmid contained in the recombinant escherichia coli carries nucleotide sequences for encoding L-tryptophan oxidase, CPA synthetase and two monooxygenases; the L-tryptophan oxidase is VioA, and the CPA synthetase is VioB. In the invention, the combination of the L-tryptophan oxidase, the CPA synthetase and the two monooxygenases can be normally expressed in escherichia coli, and the CPA and an anticancer drug intermediate can be efficiently synthesized by taking the L-tryptophan as a substrate, thereby providing a foundation for screening and research and development of the anticancer drug. |