| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_873d6a6fc681644d2da216c5a3a8f2f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d113027089608e8db37890ba62dfd28f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y501-03 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 |
| filingDate |
2019-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c4dd70ee8d1d16ed4b4019deb9e6da8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f32fc70250b3f629c55c701e31740e54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d1205f743c9c3d5ebe0016df010725c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23ed596c585af3f63a7af1c43bb13ee3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_579874091fb5f6d4b4b5771f1f761025
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bf202f8d9582e3e3a3db6b81caebadc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93b3785d5250c577ef93049c9aed725a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a124bfdcb79e245b8fea4a08b1d24532
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ef7b0e8fac4c17e3421be64fd653201 |
| publicationDate |
2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112980824-A |
| titleOfInvention |
A rapid optimization method based on heterologous expression of enzymes in different microbial cell factories |
| abstract |
The invention relates to the field of microbial engineering, in particular to a method for optimizing the heterologous expression of enzymes in a microbial cell factory. The method includes: by designing a programmed experiment method, respectively measuring the expression level of the enzyme and the growth level of the microbial cell factory under different external environmental factors , and take the highest enzyme expression level as the enzyme reference level and the highest microbial cell factory growth level as the cell reference level; select the enzyme expression level to reach at least 90% of the enzyme reference level and the microbial cell factory growth level to reach at least 70% of the cell reference level The conditions at % are optimized for heterologous expression of enzymes in microbial cell factories. The method provided by the invention has the advantages of comprehensively considering the expression characteristics of the enzyme and the growth characteristics of the host cells, and determining a qualitative and quantitative production expression method, which can realize the rapid jump from strain construction to biocatalysis process. |
| priorityDate |
2019-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |