http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112980785-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8ed828fc329e6ea7f4e26b6b2ddab6c3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-515 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 |
| filingDate | 2021-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78656cc369b05d27ab06d6aa7333a667 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c889c9a21e6125227a25949eb41a515 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4bb6261e87bdd635b878baac0fcf04d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bfeb1af2a65a302d7fcb26474901b01 |
| publicationDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112980785-A |
| titleOfInvention | A kind of preparation method of highly active tumor-infiltrating lymphocytes |
| abstract | The invention relates to the technical field of cell culture, and in particular to a method for preparing highly active tumor-infiltrating lymphocytes, comprising: step 1, treating the tumor-infiltrating lymphocyte digestion solution with collagenase, DNase and hyaluronase for processing Density gradient centrifugation; inoculation of primary tumor-infiltrating lymphocytes into complete RPMI medium for expansion; inoculation of first-generation tumor-infiltrating lymphocytes into roller bottles to continue culturing to obtain highly active tumor-infiltrating lymphocytes. The invention solves the problems of complicated preparation method and low yield of highly active tumor-infiltrating lymphocytes in the prior art. In the above preparation method, the "comprehensive enzymatic digestion method" is used to digest tissue or cell enrichment during the preparation of the tumor-infiltrating lymphocyte digestion solution, and the comprehensive enzymatic digestion can ensure the best acquisition rate of single cells. The above preparation method can be used for the preparation of clinical tumor-infiltrating lymphocytes, and a sufficient number of lymphocytes can be obtained. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116179486-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023109657-A1 |
| priorityDate | 2020-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.