| abstract |
The invention relates to an endothelial cell in vitro culture system for optimizing the working mode of continuous flow artificial heart pulsation, belonging to the technical field of artificial organs. The system includes three parts: 1) The cell culture chamber on the microfluidic chip and the off-chip multi-element aortic arch afterload hydrodynamic loop. 2) The device for simulating the power source of the cardiovascular system: the fluid loading device is realized by a pulsed blood pump; the artificial heart device is connected in parallel to both ends of the pulsed blood pump. 3) Peripheral detection and feedback control system, including pressure, flow sensor, fluorescence microscope, CCD high-speed camera system and proportional-integral-differential feedback control system. The system can accurately simulate the real hemodynamic microenvironment of vascular endothelial cells in different parts of the aortic arch, and provide miniaturization for studying the quantitative relationship between different pulsatile working modes of artificial heart pumping speed and hemodynamic signals of local arterial endothelial microenvironment. , an experimental platform for objectification, standardization and quantification. |