| abstract |
The invention discloses a nano antibody for resisting HE4, which has 3 unique complementarity determining regions CDR1, CDR2 and CDR3, and also provides an expression vector containing the coding sequence of the variable region of the nano antibody, a host cell containing the expression vector, and application of the nano antibody in detecting the content of HE4 in serum. The nano antibody provided by the invention has specific recognition and binding capacity to HE4, the affinity of the nano antibody can reach 2.773E-09, the nano antibody has a unique antigenic determinant recognition site, and excellent detection effect can be obtained in HE4 serum detection, particularly in a double antibody sandwich method. |