http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112979800-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72407e9c9d11a58e30f9fe8913909518 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-163 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 |
| filingDate | 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3aaec8f34f47a51cbb48f17d496c3e39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5827045f7c9dd26a8fbf8c33a957f5b |
| publicationDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112979800-A |
| titleOfInvention | Preparation method of paired monoclonal antibody for detecting phosphorylated tau protein |
| abstract | The invention discloses a method for preparing a paired monoclonal antibody for detecting phosphorylated tau protein. , Preparation of hybridoma cell lines, including preparation of myeloma cells, preparation of feeder cells, preparation of spleen cells, cell fusion, screening of hybridoma cells and cloning culture of hybridoma cells; the third step, the preparation of hybridoma cells Expanded culture and cryopreservation; the fourth step, the batch preparation of monoclonal antibodies; the fifth step, the purification of monoclonal antibodies; the sixth step, the identification of monoclonal antibodies, including ascites and antibody titer determination, antibody subclass identification, Antibody pairing experiment; the invention combines traditional hybridoma technology to prepare paired monoclonal antibody for detecting phosphorylated tau protein, provides a simple and effective monoclonal antibody screening preparation scheme, and continuously and stably provides necessary raw materials for diagnostic kits. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114002435-A |
| priorityDate | 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.