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filingDate 2019-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112964815-A
titleOfInvention Method for detecting multiple steroid hormones in biological body fluid
abstract The invention discloses a method for detecting multiple steroid hormones in biological body fluid, belongs to the technical field of analytical chemistry and clinical examination, and can solve the problems of long detection time, low detection efficiency and low detection sensitivity of the conventional steroid hormone detection method. Preprocessing biological body fluid containing a substance to be detected, detecting and analyzing to obtain a detection result of multiple steroid hormones in the biological body fluid; the pretreatment includes liquid-liquid extraction. According to the invention, protein and other hydrophilic micromolecular impurities are removed by extraction, so that the interference of other impurities in the sample is reduced; a non-derivatization method is adopted, so that the operation steps are simplified, and the repeatability is improved; and the content of the nine steroid hormones can be measured simultaneously, and the detection result is accurate and has good repeatability and stability.
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