http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112964772-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3272 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112964772-B |
| titleOfInvention | Method for fixing enzyme on chip based on screen printing |
| abstract | The invention relates to an enzyme immobilization method, and belongs to the technical field of enzyme biosensing. The method comprises the following specific steps: synthesizing a nano sensing material; synthesizing sensing slurry and performing carboxylation treatment; silk-screen printing of the chip; chip activation and enzyme immobilization. Based on the enzyme immobilization principle of covalent bond method, the invention prints a screen printing electrode chip with a working electrode containing a large amount of carboxyl by introducing carboxyl (-COOH) into screen printing slurry, activates the carboxyl on the electrode to have reaction activity, performs condensation reaction with amino (-NH 2) in enzyme protein, and connects and immobilizes the enzyme on the chip through covalent bond, thereby realizing the immobilization of the enzyme. The method has the advantages of simple operation and high immobilization efficiency, the enzyme activity is not influenced when the enzyme is immobilized on the screen printing chip, and the prepared enzyme electrode has good stability. |
| priorityDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.