abstract |
The culture solution comprises advanced DMEM/F12 culture medium, glucose, sodium bicarbonate, Human Serum Albumin (HSA), N2, B27, glutamine, a vitamin C, JNK inhibitor, a hedgehog pathway antagonist, an EGF signaling factor, an FGF signaling factor, a TGF-beta inhibitor, a WNT signaling pathway activator, a Notch signaling pathway inhibitor, a small molecular compound and trace elements, has different proportions in different stages of cell culture, and can efficiently induce the pluripotent stem cells to directionally differentiate into the islet beta cells. |