| abstract |
The invention discloses a preparation method of a cortical bone repairing material, which comprises the following steps of fully removing soft tissues, bone marrow and cartilage from clean heterogeneous bone materials, cleaning, degreasing, decalcifying, soaking bone blocks in glutaraldehyde solution for cross-linking treatment, soaking the bone blocks in proteolytic enzyme of pepsin for deproteinization treatment, compounding the heterogeneous bone materials with nano-silver gelatin solution, and finally freeze-drying to obtain the cortical bone grafting material. The method has the advantages that the antigenicity is reduced by the aid of degreasing, decalcification and freeze drying of the heterogeneous bone, the osteogenic activity of the heterogeneous bone is maintained, the osteogenic activity of the heterogeneous bone is improved, the deproteinization treatment is carried out by pepsin, the damage to collagen and osteogenic factors in the bone grafting material can be reduced, the nano silver micro powder adopted as an antibacterial component can have strong inhibiting and killing effects on various pathogenic microorganisms, the bone grafting material forms a slow release film, and the anti-infection effect of the heterogeneous bone grafting material can be further improved. |