http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112945667-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea798fc3adcc043e14d17a55a73e3b92 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-10 |
| filingDate | 2021-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49b6222b7133296d6625474bffe8c1a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2c2dbe6e966baea1e805c68bf08b342 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ed968a2baccb01e6ff1b561c5f45931 |
| publicationDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112945667-A |
| titleOfInvention | Preparation method of blood single cell suspension for removing nucleated red blood cells |
| abstract | The invention discloses a preparation method of a blood single cell suspension for removing nucleated red blood cells, which comprises the steps of pumping fresh nucleated red blood cell blood into an enzyme-free sterile centrifuge tube containing 50uL EDTA-K2, incubating for 10min at 20 ℃, and centrifuging to remove blood plasma; adding a precooled nucleated erythrocyte lysate into a centrifuge tube, incubating, centrifuging and discarding a supernatant; adding a precooled nucleated erythrocyte lysate into a centrifugal tube, incubating, centrifuging and discarding a supernatant; and adding enzyme-free sterile PBS into the centrifuge tube, detecting the cell concentration by using a cell counter or a blood counting plate, calculating the cell viability by trypan blue staining, and adding the enzyme-free sterile PBS with a proper volume for diluting the single cell suspension to ensure that the final cell concentration meets the operation on a computer of 10 Xgenomics. The method can realize low-cost, quick and efficient obtaining of the blood single cell suspension from which the nucleated red blood cells are removed, and ensures that other blood cells keep good activity. |
| priorityDate | 2021-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.