| abstract |
The invention belongs to the technical field of biology, and particularly relates to a lysate for extracting DNA from peanut leaves and an extraction method of the DNA from the peanut leaves. The lysis solution for extracting the DNA in the peanut leaves comprises the following components: 1.5% CTAB, 90-110 mM Tris-HCl, 15-25 mM EDTA, 1M KCl, and adjusting the pH value to 8.0. The DNA of the peanut leaves extracted by the lysate has no viscosity, no impurities and pigments, clear main band and low degradation degree; the method can be used for high-quality mass DNA extraction of peanut leaf tissues, and meets the requirement of high-quality mass DNA extraction of peanut population samples in peanut molecular breeding. |