| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3a96160327a9469cab036f765f319768 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01041
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2457
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-44 |
| filingDate |
2021-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16115a4071c499adba20b78e940a5e89
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1d6c9f0c45617f0dc45e4d2e62912dd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b97c5a29baa5a947a9fc441ecfaf987
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53107ec3cb76716d7c2f490b36867459 |
| publicationDate |
2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112941056-A |
| titleOfInvention |
A starch pullulanase mutant and its application |
| abstract |
The invention discloses a starch pullulanase mutant and an application thereof. The method for obtaining the mutant is the starch pullulanase SmApu parent enzyme derived from the archaea Staphylothermus marinus F1, and the phenylalanine or phenylalanine at the 395th position is changed. A starch pullulanase mutant obtained by mutating phenylalanine at position 510. The starch pullulanase mutant of the present invention has the activity of hydrolyzing α-1,6-glycosidic bonds in amylopectin, but completely loses the activity of hydrolyzing cyclodextrin. In the high temperature reaction system, adding the starch pullulanase mutant of the present invention can increase the total yield of cyclodextrin by more than 43%. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112941056-B |
| priorityDate |
2021-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |