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filingDate 2021-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a10c168d61011b6c7f9c8eb816ebfca
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publicationDate 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112941046-A
titleOfInvention A kind of production technology and production equipment of high activity and high specificity fructosyltransferase
abstract The invention relates to the technical field of fructosyltransferase production, and specifically discloses a production process and production equipment of a high-activity and high-specificity fructosyltransferase; the invention comprises the following steps: taking an appropriate amount of Aspergillus oryzae and inoculating it into a fructo-oligosaccharide-based matrix. Activation on the slant medium; high-temperature steam sterilization in the culture tank; the activated strain slant is made into spore liquid with physiological saline and the culture medium is added to the culture tank for cultivation; centrifugation is performed after fermentation; Concentrate, and finally spray-dry the prepared concentrated solution; the production equipment includes a culture tank, a centrifugal separation tank and an ultrafiltration membrane concentration device; the invention discloses the use of fructooligosaccharide as a matrix for activation of the culture medium, and the culture medium is cultured during the culture process. The nutrient source in the liquid is also fructooligosaccharide, which can induce the Aspergillus oryzae in the process of culturing, so that the fructosyltransferase obtained by the Aspergillus oryzae transformation can have higher activity and better specificity.
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type http://data.epo.org/linked-data/def/patent/Publication

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