http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112940125-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57484 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2827 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 |
filingDate | 2021-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112940125-B |
titleOfInvention | anti-VISTA protein monoclonal antibody, cell strain thereof, preparation method and application |
abstract | The invention relates to a monoclonal antibody capable of identifying a human VISTA antigen, a secretory cell strain, a preparation method thereof and application thereof in immunoassay. According to the technical scheme, 269 th to 289 th amino acids at the tail end of the VISTA protein are selected as antigen peptides, the mouse is immunized by immunogen obtained after coupling KLH, and a mouse hybridoma cell strain 11A4 capable of efficiently secreting the anti-VISTA protein monoclonal antibody and the anti-VISTA protein monoclonal antibody secreted by the cell strain are obtained through cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing VISTA protein, and is suitable for immunological detection, particularly immunohistochemical detection. |
priorityDate | 2021-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.